Competences
Physiology: Measure intracellular concentrations in inorganic ions by spectrometry; osmometry; perfusion of shellfish gills; microinjection of biological substances (nucleic proteins and acids) or of drugs in ovocytes of Amphibians; handling of the radioactivity (14C, 32P, 3H, 125I); insulation of the mitochondries of various fabrics (liver, kidney, muscle); oxygraphy, measurement of the calcic holding capacity and the production of free radicals on cells whole, permeabilized, and on mitochondriales fractions.
Biochemistry and molecular biology: fractionation subcellulaire in gradient sucrose; electrophoresis SDS-PAGE with a dimension (colourings with the money and the blue of Coomassie); autoradiography; radioimmunoassay; Western Blot (detection by colorimetry and chémiluminescence); separation of nucleotides by HPLC; synthesis ARN messengers starting from the cDNA; enzymatic proportionings by spectrophotometry or fluorimetry; protein proportionings (Biuret, Bradford, Lowry, Pierce).
Cellular culture: culture of micro-organisms (following bacterial stocks: Pseudomonas, Arthrobacter, Bacillus) out of flasks or small fermentors; cellular cultures of immortalized lines (KB, HMEC-1, U937, K562, C6, HL-1, Caco-2) and of primary cells (BAEC, HUVEC, neurons, astrocytes); determination of cellular viability (test of exclusion to blue trypan, double marking iodizes propidium+annexineV with the cytometer of flow).
Microscopy: optics, contrast of phase, épifluorescence and fluorescence confocale; immunocytochimy: specific marking of the mitochondries by autofluorescence (NADH, FAD) or via the use of exogenic probes (TMRM, mitotracker, calcein/cobalt, mitoSOX, DCF-DA).
Handling of the animals: Wistar rats (nourished, with jeûn); injection into subcutaneous or intrapéritonéale of various agents (anaesthetic, medicamentous drugs).
Other competences: drafting of articles, scientific projects, management, and oral presentation reports of the results (see list of the publications and participation in the national and international congresses in appendix); revision of articles.
Use of L \ \ \ \ \ \ \ 'data-processing tool (resourcefulness \ \ \ \ \ \ \ 'Windows exploitation, software D \ \ \ \ \ \ \ 'imagery)

Experiment
# 04/2007 - At the end of 2008: Researcher associated with Université with Salamanque, Spain
Scientific collaboration on various projects: (Directors: Professors Yehia El-Mir and J.M. Lopez-Novoa)
has) Metformine and nephrotoxicity induced by gentamicin: studies in vivo.
b) Silibinine and the hepatic metabolism mitochondrial: nutritional models of resistance to insulin and/or oxydative stress (project financed by the Spanish Ministry of Education and Sciences).
c) Study of the acute hepatic effects of the glitazones (project financed by the Spanish Ministry of Health and Consumption (Funds of medical investigation)
1 publication, 4 abstracts published
Framing of 2 students in thesis, planning of new experimental sets.
# 08/2002 - 03/2007: Post-doctorate at the University Joseph Fourier of Grenoble, France
Titrate: Role of the metformine and some derived in control from the cellular death induced by an oxydative stress (Financing by the pharmaceutical group Merck-Health, Lyon, France)
8 articles, 2 oral communications, 5 abstracts including 3 published
Team work within a Unit INSERM (U884, Director: Xavier Leverve) with multiple collaborations and international partnerships.
Co-animation of a workshop for the Festival of Science (24-25/10/05), regular training of methodologies to trainees
# 05/1998 - 07/2002: Doctorate at the University of Namur, within the Unit of Search in Biology of the Organizations (Laboratory of compared Biochemistry and Physiology)
Thesis financed completely by the pharmaceutical group Merck-Health, Lyon, France.
3 articles, 1 oral communication
Framing of a coed for her report
# 06/1995 - 04/1998: Researcher in Université of Namur. CDD in convention with the pharmaceutical group Merck-Lipha: Identification of the cellular and molecular mechanisms by which the metformine stimulates the metabolism of glucose insulino-dependent in the ovocyte on Xenopus laevis.
1 publication, 3 abstracts including 2 published
# 03/1994 - 06/1994: Researcher in Université of Namur. CDD with the Walloon Area and the University of Liege (Study of the hormonal factors similar to prolactins of cructacé and the somatotropine)

Formations
2002 - Doctor of Biology
University of Namur (FUNDP), Belgium
Titrate: L \ \ \ \ \ \ \ 'oocyte of Xenopus laevis like experimental support for L \ \ \ \ \ \ \ 'study D \ \ \ \ \ \ \ 'a antidiabetic substance, the metformine, intervening in the control of the glucidic metabolism by L \ \ \ \ \ \ \ 'insulin.
1993 - Bachelor in Biochemistry
University of Liege, Belgium
Titrate: Study of degradation by microbial way of recalcitrant aromatic compounds contained in fuel oil. 1 abstract
1991 - Bachelor in Zoological Sciences
University of Namur (FUNDP), Belgium
Titrate: Fructose-2,6-bisphosphate and bioamines in ionic transport on the level of the gills of the crab Eriocheir sinensis acclimatized to L \ \ \ \ \ \ \ 'fresh water. 1 article

Practised languages
- French (mother tongue)
- English (good level)
- Spaniard (operational, in training)
- Netherlander (school concepts)

Availability
January 2009

Current remuneration
1500 euros